, 1758): A Field Study on the Side Effects of Methyltestosterone. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. doi:10.4172/2155-9546.1000416 Copyright: © 2016 Sayed AEDH, et al.Achievement of high growth rate, prevention of large energy dissipation into reproduction and courtship behaviour, reducing aggressiveness, uniformity in size, avoiding undesirable effects of sexual maturation on appearance and meat quality, and reducing undesirable environmental impacts are the main contributory factors in rising the interest about monosex fish cultures  One of the most practiced sex reversal techniques is hormonal induction especially using methyltestosterone that has been test in more than 25 fish species .However, this technique faces several limitations and disadvantages like time and cost consumption, low survival of sex reversed male or female, delayed sexual maturity, sterility at high dose, paradoxical sex reversal, and carcinogenicity of hormonal residues and other health hazards [5,6].The mean values of the absorbance were obtained for the standards and the samples were divided by the absorbance value for the first standard (zero standard) and multiplied by 100.The zero standard was made equal to 100%, and the absorbance values were quoted in percentages.Serum TPX content of the monosex fishes obtained from Alexandria farms were significantly higher than the wild fishes obtained from Assiut farms.
The wells were filled three times, each time with 250 μl of distilled water and the liquid was poured out as the previous step.
It adversely impacted fish welfare on several levels as growth  reproduction , and immunity .
Two face impacts of androgenic steroids on redox homeostatic status is emerged from literature.
Oxidative stress is a situation characterized by an imbalance between increased production of oxidant species and/or decreased efficacy of the antioxidant defense system  leading acromolecule to damage including lipid peroxidation, protein crosslinking, DNA damage, changes in growth and function of cells .
The investigation of this imbalance is difficult due to the limited availability of specific biomarkers of oxidative stress, and the fact that measurement of individual antioxidant may give misleading picture because antioxidants work in concert through chain breaking reactions.
Therefore, analysis of total antioxidant capacity may be the most relevant investigation  and take into consideration the cumulative synergistic action of all the antioxidants present in the sample providing an integrated parameter rather than the simple sum of measurable antioxidants .